Electrophoresis:Article Title: Soluble expression of protein using peptide tag
Article Snippet: For electrophoresis (SDS-PAGE) of protein, an electrophoresis tank (Criterion cell, BIO RAD) and Criterion TGX-gel (BIO RAD) were used. .. In the electrophoresis tank, an electrophoresis buffer (Tris/Glycine/SDS Buffer, BIO RAD) was placed, and 4 μl of the SDS-treated sample was applied to each well, followed by performing electrophoresis at a constant voltage of 200 V for 40 minutes. .. The gel after the electrophoresis was subjected to blotting by Trans-Blot Turbo (BIO RAD) using a Trans-Blot Transfer Pack (BIO RAD).
Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
Article Snippet: Then, the samples were sonicated and centrifuged, and mPAGE 4X LDS Sample Buffer supplemented with 50 mM β-mercaptoethanol (Merck Life Science (Poznań, Poland)) was used as a sample buffer. .. A total of 30 μg of proteins was loaded into each lane of the mPAGE Bis-Tris gels, and then, the proteins were separated by SDS polyacrylamide gel electrophoresis at 180 V for 30 min in an electrophoresis tank (Mini-PROTEAN Tetra Cell, Bio-Rad, Hercules, CA, USA) using MOPS SDS running buffer, Merck Life Science (Poznań, Poland). .. Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions.
Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
Article Snippet: Then, the samples were sonicated and centrifuged, and mPAGE 4X LDS Sample Buffer supplemented with 50 mM β-mercaptoethanol (Merck Life Science (Poznań, Poland)) was used as a sample buffer. .. A total of 30 μg of proteins was loaded into each lane of the mPAGE Bis-Tris gels, and then, the proteins were separated by SDS polyacrylamide gel electrophoresis at 180 V for 30 min in an electrophoresis tank (Mini-PROTEAN Tetra Cell, Bio-Rad, Hercules, CA, USA) using MOPS SDS running buffer, Merck Life Science (Poznań, Poland). .. Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions.
Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Article Title: A clinical grade neurostimulation implant for hierarchical control of physiological activity
Article Snippet: .. The platform consists of an electrophoresis tank (Bio-Rad Laboratories Inc., Hercules, CA, US) filled with isotonic saline (0.9% (w/v)) and a custom hardware interface board. ..
Article Title: miR-1 Promotes Apoptosis and Aggravates Myocardial Ischemia–Reperfusion Injury by Downregulating Insulin-Like Growth Factor-1
Article Snippet: Joklik (Sigma, St. Louis, Missouri, United States, Cat# M0518-10L). .. Western blotting system, gel imaging system, electrophoresis instrument, and electrophoresis tank (Bio-rad, United States). .. Thermo Forma 3111 carbon dioxide incubator (Thermo Fisher Scientific, United States).
Article Title: Gastrointestinal stability, antimicrobial retention, and gut microbiota modulation by bovine lactoferrin: An integrated in vitro and vivo study
Article Snippet: Lactoferrin, a multifunctional glycoprotein, exhibits diverse biological activities including antibacterial, antiinflammatory, and immunomodulatory properties, demonstrating significant potential for development in food and biomedical industries.. However, the stability of lactoferrin in the gastrointestinal and its effect on gut microbiota have not been elucidated, which hindered its clinical translation.. This study systematically investigated the degradation dynamics of bovine lactoferrin (bLF) under simulated gastric fluid (SGF) and acidic conditions via Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), while evaluating the antibacterial efficacy of its hydrolysates against Helicobacter pylori (H. pylori) in vitro.
Polyacrylamide Gel Electrophoresis:Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
Article Snippet: Then, the samples were sonicated and centrifuged, and mPAGE 4X LDS Sample Buffer supplemented with 50 mM β-mercaptoethanol (Merck Life Science (Poznań, Poland)) was used as a sample buffer. .. A total of 30 μg of proteins was loaded into each lane of the mPAGE Bis-Tris gels, and then, the proteins were separated by SDS polyacrylamide gel electrophoresis at 180 V for 30 min in an electrophoresis tank (Mini-PROTEAN Tetra Cell, Bio-Rad, Hercules, CA, USA) using MOPS SDS running buffer, Merck Life Science (Poznań, Poland). .. Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions.
Article Title: Liposomal Vitamin C as a Modulator of the Efficacy of Ceralasertib Therapy in Ovarian Cancer
Article Snippet: Then, the samples were sonicated and centrifuged, and mPAGE 4X LDS Sample Buffer supplemented with 50 mM β-mercaptoethanol (Merck Life Science (Poznań, Poland)) was used as a sample buffer. .. A total of 30 μg of proteins was loaded into each lane of the mPAGE Bis-Tris gels, and then, the proteins were separated by SDS polyacrylamide gel electrophoresis at 180 V for 30 min in an electrophoresis tank (Mini-PROTEAN Tetra Cell, Bio-Rad, Hercules, CA, USA) using MOPS SDS running buffer, Merck Life Science (Poznań, Poland). .. Next, the proteins were transferred onto 0.45 μm PVDF membranes using a semi-dry transfer Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) in mPAGE Transfer Buffer according to the mPAGE Bis-Tris gel manufacturer’s optimized instructions.
Transferring:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Membrane:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Polymerase Chain Reaction:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Real-time Polymerase Chain Reaction:Article Title: METTL7A drives pituitary neuroendocrine tumor invasion via an m6A-dependent epigenetic mechanism upregulating EGFR/PI3K/Akt signaling
Article Snippet: .. T25 flasks (TCF012050), T75 flasks (TCF012250), 6 well-plates (TCP011006), 15 mL centrifuge tubes (CFT920150), 50 mL centrifuge tubes (CFT920500) (Jet BIOFIL, China); Non-enzymatic pipette tips (KeyGEN, China); 1.5 mL Non-enzymatic centrifuge tube (Axygen, China); PVDF membrane (IPVH00010) (Sigma-Aldrich, America) thin plate of a glass plate electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); pipettes (Eppendorf Middle East & Africa GmbH, Germany); nitrile latex gloves (Loctite); Thin plate of glass plate of electrophoresis tank (1653308), thick plate of glass plate of electrophoresis tank (1653311) (Bio-Rad, USA); Transwell chamber (8 μm model: 3422) (CORNING, China); 0.2 mL PCR single tube (PCR-02C) (Axygen, China); 0.1 mL qPCR eight-strip tube (TLS0851) (BIO-RAD, USA); Fluorometric 96-well PCR plate (F603102), fluorometric nuclease-free mounting (F604419) (Sangon, China). .. Fetal bovine serum (FBS, #FSD500; Excell Bio, China); NonPreservative Penicillin-Streptomycin Solution (100x, #C0222; Beyotime, China); DMEM High Glucose, #11965118; Gibco, MA, USA); Trypsin (#R001100; Gibco, CardioGiren, China); Cell Freezing Medium (#C0210B-50 ml), PBS Buffer (#C0221A), APS (#ST005), RIPA Lysis Buffer (#P0013B), PMSF (#ST506), TEMED (#ST728; Beyotime, China); 30% Acrylamide/Bis Solution (#BL513A); Tris-Base (#BS083); TBS Buffer Powder (#BL602A); BSA Protein Standard (#BL673A); Tween-20 (#BS100; Biosharp, China); SDS (#3250); Glycine (#1275); Non-fat Milk Powder (#1172; Biolfroxx, Germany); BCA Protein Assay Kit (#WB6501), 5 × SDS-PAGE Loading Buffer (#WB2001), ECL Substrate (#P2100; NCM Biotech, Shanghai, China); methanol (#HB05; Guangzhou Chemical Reagent, China); prestained protein marker II (#G2058 - 250UL; Servicebio, China); GAPDH (14C10) rabbit mAb (#2118; Cell Signaling Technology, USA); Anti-METTL7A (ab79207, Abcam, UK), Anti-EGFR Antibody [EP38y] (ab52894, Abcam, UK), PI3K p85 alpha (U5) (Thermo Fisher, USA), Phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher, USA), Akt (#9272, CST, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (#4060, CST, USA), Anti-GAPDH (6C5) (#ab8245, Abcam, UK), HRP-conjugated Goat Anti-rabbit (bs0295G-HRP, Bioss, China), HRP-Goat Anti-Mouse IgG (bs-0296G-HRP, Bioss, China); Lipofectamin 3000 (L3000015, Thermo Fisher, USA); Cell/Tissue Total RNA Extraction Kit V2 (RC112, Vazyme, China); HiScript III 1st strand cDNA Synthesis Kit (R312, Vazyme, China); Taq Pro Universal SYBR qPCR Master Mix (Q712, Vazyme, China); PCR Primer (Sangon Biotech, China); CCK-8 Cell Proliferation Assay Kit (BA00208, Bioss, China); BD Matrigel (354230, Corning, USA); 4 % Paraformaldehyde (P0099, Beyotime, China); 1 % Crystal Violet (V5265-250 ML, Sigma-Aldrich, USA); BeyoClickTM EdU-488 Cell Proliferation Kit (C0071S, Beyotime, China); RNA Immunoprecipitation (RIP) Kit (Bes5101(N), Bersin.
Saline:Article Title: A clinical grade neurostimulation implant for hierarchical control of physiological activity
Article Snippet: .. The platform consists of an electrophoresis tank (Bio-Rad Laboratories Inc., Hercules, CA, US) filled with isotonic saline (0.9% (w/v)) and a custom hardware interface board. ..
Western Blot:Article Title: miR-1 Promotes Apoptosis and Aggravates Myocardial Ischemia–Reperfusion Injury by Downregulating Insulin-Like Growth Factor-1
Article Snippet: Joklik (Sigma, St. Louis, Missouri, United States, Cat# M0518-10L). .. Western blotting system, gel imaging system, electrophoresis instrument, and electrophoresis tank (Bio-rad, United States). .. Thermo Forma 3111 carbon dioxide incubator (Thermo Fisher Scientific, United States).
Imaging:Article Title: miR-1 Promotes Apoptosis and Aggravates Myocardial Ischemia–Reperfusion Injury by Downregulating Insulin-Like Growth Factor-1
Article Snippet: Joklik (Sigma, St. Louis, Missouri, United States, Cat# M0518-10L). .. Western blotting system, gel imaging system, electrophoresis instrument, and electrophoresis tank (Bio-rad, United States). .. Thermo Forma 3111 carbon dioxide incubator (Thermo Fisher Scientific, United States).
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